Journal Articles ES Journal of Dental Sciences Year : 2023

Effects of ethylenediaminetetraacetic acid on stem cells from the apical papilla: In vitro study

Abstract

Ethylenediaminetetraacetic acid (EDTA) is used as an irrigant in regenerative endodontic treatment. The present study aimed to investigate the effects of EDTA on stem cells from apical papilla (SCAPs) in vitro.
Overall, SCAPs exhibited mesenchymal stem cell characteristics. EDTA treatment at 2.50% and 1.25% did not significantly exhibit cytotoxicity and alter cell morphology. However, EDTA attenuated cell proliferation and reduced MKI67 mRNA expression in SCAPs. Further, EDTA significantly induced early cell apoptosis at 48 h. Cell migration was delayed with EDTA treatment. After maintaining SCAPs in an osteogenic induction medium, EDTA diminished mineral deposition by SCAPs on day 14.
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hal-04233769 , version 1 (08-01-2025)

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Suphalak Phothichailert, Benya Sangwisutsai, Dhitinant Rattanakosol, Nutcha Teerapongpaibul, Sirawut Hiran-Us, et al.. Effects of ethylenediaminetetraacetic acid on stem cells from the apical papilla: In vitro study. ES Journal of Dental Sciences , 2023, 18 (1), pp.50-56. ⟨10.1016/j.jds.2022.07.014⟩. ⟨hal-04233769⟩
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