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Initiation of cyp26a1 Expression in the Zebrafish Anterior Neural Plate by a Novel Cis-Acting Element

Chunhong Chen 1 Aline Stedman 1 Emmanuelle Havis 1 Isabelle Anselme 1 Daria Onichtchouk 2 François Giudicelli 1, * Sylvie Schneider-Maunoury 1, *
* Corresponding author
1 LBD-E10 - Morphogénèse du Cerveau des Vertébrés = Morphogenesis of the vertebrate brain
LBD - Laboratoire de Biologie du Développement, INSERM - Institut National de la Santé et de la Recherche Médicale : U1156
Abstract : Early patterning of the vertebrate neural plate involves a complex hierarchy of inductive interactions orchestrated by signalling molecules and their antagonists. The morphogen ret-inoic acid, together with the Cyp26 enzymes which degrade it, play a central role in this process. The cyp26a1 gene expressed in the anterior neural plate thus contributes to the fine modulation of the rostrocaudal retinoic acid gradient. Despite this important role of cyp26a1 in early brain formation, the mechanisms that control its expression in the anterior neural plate are totally unknown. Here, we present the isolation of a 310-base-pair DNA element adjacent to cyp26a1 promoter, displaying enhancer activity restricted to the anterior neural plate of the zebrafish gastrula. We show that unlike that of cyp26a1, expression driven by this cyp26a1 anterior neural plate element (cANE) is independent of retinoic acid. Through deletion analysis, we identify a 12-nucleotide motif essential for cANE activity. A consensus bipartite binding site for SoxB:Oct transcription factors overlaps with this motif. Mutational analysis suggests that SoxB binding is essential for its activity. We discuss the contribution of this study to the elucidation of the regulatory hierarchy involved in early neural plate patterning.
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Chunhong Chen, Aline Stedman, Emmanuelle Havis, Isabelle Anselme, Daria Onichtchouk, et al.. Initiation of cyp26a1 Expression in the Zebrafish Anterior Neural Plate by a Novel Cis-Acting Element. PLoS ONE, Public Library of Science, 2016, 11 (3), pp.e0150639. ⟨10.1371/journal.pone.0150639⟩. ⟨hal-01300689⟩

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