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Core–Shell Pure Collagen Threads Extruded from Highly Concentrated Solutions Promote Colonization and Differentiation of C3H10T1/2 Cells

Abstract : The elaboration of scaffolds able to efficiently promote cell differentiation toward a given cell type remains challenging. Here, we engineered dense type I collagen threads with the aim of providing scaffolds with specific morphological and mechanical properties for C3H10T1/2 mesenchymal stem cells. Extrusion of pure collagen solutions at different concentrations (15, 30, and 60 mg/mL) in a PBS 5× buffer generated dense fibrillated collagen threads. For the two highest concentrations, threads displayed a core-shell structure with a marked fibril orientation of the outer layer along the longitudinal axis of the threads. Young's modulus and ultimate tensile stress as high as 1 and 0.3 MPa, respectively, were obtained for the most concentrated collagen threads without addition of any cross-linkers. C3H10T1/2 cells oriented themselves with a mean angle of 15-24° with respect to the longitudinal axis of the threads. Cells penetrated the 30 mg/mL scaffolds but remained on the surface of the 60 mg/mL ones. After three weeks of culture, cells displayed strong expression of the tendon differentiation marker Tnmd, especially for the 30 mg/mL threads. These results suggest that both the morphological and mechanical characteristics of collagen threads are key factors in promoting C3H10T1/2 differentiation into tenocytes, offering promising levers to optimize tissue engineering scaffolds for tendon regeneration.
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https://hal.archives-ouvertes.fr/hal-03113880
Contributor : Gervaise Mosser Connect in order to contact the contributor
Submitted on : Wednesday, March 10, 2021 - 3:50:07 PM
Last modification on : Thursday, October 21, 2021 - 4:02:38 PM
Long-term archiving on: : Friday, June 11, 2021 - 7:04:17 PM

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Lise Picaut, Lea Trichet, Christophe Hélary, Guillaume Ducourthial, Marie-Ange Bonnin, et al.. Core–Shell Pure Collagen Threads Extruded from Highly Concentrated Solutions Promote Colonization and Differentiation of C3H10T1/2 Cells. ACS Biomaterials Science and Engineering, ACS, 2021, ⟨10.1021/acsbiomaterials.0c01273⟩. ⟨hal-03113880⟩

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