Skip to Main content Skip to Navigation
Journal articles

Role of the DHH1 Gene in the Regulation of Monocarboxylic Acids Transporters Expression in Saccharomyces cerevisiae

Abstract : Previous experiments revealed that DHH1, a RNA helicase involved in the regulation of mRNA stability and translation, complemented the phenotype of a Saccharomyces cerevisiae mutant affected in the expression of genes coding for monocarboxylic-acids transporters, JEN1 and ADY2 (Paiva S, Althoff S, Casal M, Leao C. FEMS Microbiol Lett, 1999, 170:301– 306). In wild type cells, JEN1 expression had been shown to be undetectable in the presence of glucose or formic acid, and induced in the presence of lactate. In this work, we show that JEN1 mRNA accumulates in a dhh1 mutant, when formic acid was used as sole carbon source. Dhh1 interacts with the decapping activator Dcp1 and with the deadenylase complex. This led to the hypothesis that JEN1 expression is post-transcriptionally regulated by Dhh1 in formic acid. Analyses of JEN1 mRNAs decay in wild-type and dhh1 mutant strains confirmed this hypothesis. In these conditions, the stabilized JEN1 mRNA was associated to polysomes but no Jen1 protein could be detected, either by measurable lactate carrier activity, Jen1-GFP fluorescence detection or western blots. These results revealed the complexity of the expression regulation of JEN1 in S. cerevisiae and evidenced the importance of DHH1 in this process. Additionally, microarray analyses of dhh1 mutant indicated that Dhh1 plays a large role in metabolic adaptation, suggesting that carbon source changes triggers a complex interplay between transcriptional and post-transcriptional effects.
Document type :
Journal articles
Complete list of metadata

Cited literature [89 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-01527344
Contributor : Gestionnaire Hal-Upmc Connect in order to contact the contributor
Submitted on : Wednesday, May 24, 2017 - 12:01:06 PM
Last modification on : Monday, January 10, 2022 - 4:38:02 PM
Long-term archiving on: : Monday, August 28, 2017 - 12:46:47 AM

File

journal.pone.0111589.PDF
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Sandra Mota, Neide Vieira, Sónia Barbosa, Thierry Delaveau, Claire Torchet, et al.. Role of the DHH1 Gene in the Regulation of Monocarboxylic Acids Transporters Expression in Saccharomyces cerevisiae. PLoS ONE, Public Library of Science, 2014, 9 (11), pp.e111589. ⟨10.1371/journal.pone.0111589⟩. ⟨hal-01527344⟩

Share

Metrics

Les métriques sont temporairement indisponibles