Skip to Main content Skip to Navigation
Journal articles

Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome

Abstract : Catenation links between sister chromatids are formed progressively during DNA replica-tion and are involved in the establishment of sister chromatid cohesion. Topo IV is a bacterial type II topoisomerase involved in the removal of catenation links both behind replication forks and after replication during the final separation of sister chromosomes. We have investigated the global DNA-binding and catalytic activity of Topo IV in E. coli using genomic and molecular biology approaches. ChIP-seq revealed that Topo IV interaction with the E. coli chromosome is controlled by DNA replication. During replication, Topo IV has access to most of the genome but only selects a few hundred specific sites for its activity. Local chro-matin and gene expression context influence site selection. Moreover strong DNA-binding and catalytic activities are found at the chromosome dimer resolution site, dif, located opposite the origin of replication. We reveal a physical and functional interaction between Topo IV and the XerCD recombinases acting at the dif site. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle.
Complete list of metadata

Cited literature [43 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-01527413
Contributor : Gestionnaire Hal-Upmc Connect in order to contact the contributor
Submitted on : Wednesday, May 24, 2017 - 1:31:22 PM
Last modification on : Wednesday, November 17, 2021 - 12:31:52 PM
Long-term archiving on: : Monday, August 28, 2017 - 12:39:12 AM

File

journal.pgen.1006025.PDF
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Hafez El Sayyed, Ludovic Le Chat, Elise Lebailly, Elise Vickridge, Carine Pages, et al.. Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome. PLoS Genetics, Public Library of Science, 2016, 12 (5), pp.e1006025. ⟨10.1371/journal.pgen.1006025⟩. ⟨hal-01527413⟩

Share

Metrics

Record views

436

Files downloads

377