The translational repressor 4E-BP called to order by eIF4E: new structural insights by SAXS.
Résumé
eIF4E binding protein (4E-BP) inhibits translation of capped mRNA by binding to the initiation factor eIF4E and is known to be mostly or completely unstructured in both free and bound states. Using small angle X-ray scattering (SAXS), we report here the analysis of 4E-BP structure in solution, which reveals that while 4E-BP is intrinsically disordered in the free state, it undergoes a dramatic compaction in the bound state. Our results demonstrate that 4E-BP and eIF4E form a 'fuzzy complex', challenging current visions of eIF4E/4E-BP complex regulation.
Domaines
Biochimie, Biologie MoléculaireOrigine | Fichiers éditeurs autorisés sur une archive ouverte |
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