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Article Dans Une Revue Analyst Année : 2013

Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles

Résumé

We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).
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Dates et versions

hal-01044451 , version 1 (23-07-2014)

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Jolanda Spadavecchia, Ramesh Perumal, Alexandre Barras, Joël Lyskawa, Patrice Woisel, et al.. Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles. Analyst, 2013, 139, pp.157-164. ⟨10.1039/c3an01794j⟩. ⟨hal-01044451⟩
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