Background Kymograph analysis is a method widely used by researchers to analyze particle dynamics in one dimensional (1D) trajectories. Results Here we provide a Visual Basic-coded algorithm to use as a Microsoft Excel add-in that automatically analyzes particles in 2D trajectories with all the advantages of kymograph analysis. Conclusions This add-in, which we named SkyPad, leads to significant time saving and higher accuracy of particle analysis. Finally, SkyPad can also be used for 3D trajectories analysis.