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The Matrix protein M1 from influenza C virus induces tubular membrane invaginations in an in vitro cell membrane model

Abstract : Matrix proteins from enveloped viruses play an important role in budding and stabilizing virus particles. In order to assess the role of the matrix protein M1 from influenza C virus (M1-C) in plasma membrane deformation, we have combined structural and in vitro reconstitution experiments with model membranes. We present the crystal structure of the N-terminal domain of M1-C and show by Small Angle X-Ray Scattering analysis that full-length M1-C folds into an elongated structure that associates laterally into ring-like or filamentous polymers. Using negatively charged giant unilamellar vesicles (GUVs), we demonstrate that M1-C full-length binds to and induces inward budding of membrane tubules with diameters that resemble the diameter of viruses. Membrane tubule formation requires the C-terminal domain of M1-C, corroborating its essential role for M1-C polymerization. Our results indicate that M1-C assembly on membranes constitutes the driving force for budding and suggest that M1-C plays a key role in facilitating viral egress.
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https://hal.sorbonne-universite.fr/hal-01466643
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Submitted on : Monday, February 13, 2017 - 4:27:48 PM
Last modification on : Monday, December 14, 2020 - 9:56:50 AM

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David Saletti, Jens Radzimanowski, Grégory Effantin, Daniel Midtvedt, Stéphanie Mangenot, et al.. The Matrix protein M1 from influenza C virus induces tubular membrane invaginations in an in vitro cell membrane model. Scientific Reports, Nature Publishing Group, 2017, 7, pp.40801. ⟨10.1038/srep40801⟩. ⟨hal-01466643⟩

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