B- and T-cell subpopulations in patients with severe idiopathic membranous nephropathy may predict an early response to rituximab - Sorbonne Université Accéder directement au contenu
Article Dans Une Revue Kidney International Année : 2017

B- and T-cell subpopulations in patients with severe idiopathic membranous nephropathy may predict an early response to rituximab

Résumé

Primary membranous nephropathy (PMN) is characterized by antibodies to the podocyte, but little is known about B- and T-cell populations and their response to rituximab is controversial. To help resolve this we compared 33 lymphocyte subpopulations and 27 cytokines/chemokines in 25 patients with severe PMN and 27 age-matched healthy individuals. At baseline, patients had a significantly increased percentage of naive B-cells with significantly decreased switched and non-switched memory B-cells. There was a significantly decreased percentage of natural killer (NK) cells with an increase in the CD56brightCD16-/lo NK subset. There were a significantly decreased percentage of regulatory T cells, together with an increased plasma concentration of TNF-alpha, IL-5 and IL-2RA. We then investigated 16 patients at eight days and three and six months after treatment with rituximab added to supportive therapy compared to nine patients with supportive therapy alone. After rituximab, B-cell recovery was still incomplete at six months, with persistent alterations of B-cell subsets, a significant increase of both T-regulatory (Treg) cells and NK cells, and a significant decrease of both the CD56brightCD16-/lo NK subset and TNF-alpha levels. The patients who clinically responded to rituximab had a significantly lower percentage of Tregs at baseline compared to non-responders and a significantly increased percentage at day eight. Tregs remained unchanged in non-responders and in patients treated with supportive therapy alone. Thus, evaluation of Tregs might be useful for predicting early response to rituximab.
Fichier principal
Vignette du fichier
Rosenzwajg_B-_and_T-cell.pdf (1.11 Mo) Télécharger le fichier
Treg Suppl revised .pdf (367.31 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01493184 , version 1 (23-03-2017)

Identifiants

Citer

Michelle Rosenzwajg, Eva Languille, Hanna Debiec, Joana Hygino, Karine Dahan, et al.. B- and T-cell subpopulations in patients with severe idiopathic membranous nephropathy may predict an early response to rituximab. Kidney International, 2017, pp.227-237. ⟨10.1016/j.kint.2017.01.012⟩. ⟨hal-01493184⟩
180 Consultations
702 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More