Lagrangian 3D tracking of fluorescent microscopic objects in motion

Abstract : We describe the development of a tracking device, mounted on an epi-fluorescent inverted microscope, suited to obtain time resolved 3D Lagrangian tracks of fluorescent passive or active micro-objects in micro-fluidic devices. The system is based on real-time image processing, determining the displacement of a x,y mechanical stage to keep the chosen object at a fixed position in the observation frame. The z displacement is based on the refocusing of the fluorescent object determining the displacement of a piezo mover keeping the moving object in focus. Track coordinates of the object with respect to the micro-fluidic device, as well as images of the object are obtained at a frequency of several tenths of Hertz. This device is particularly well adapted to obtain trajectories of motile micro-organisms in micro-fluidic devices with or without flow.
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Review of Scientific Instruments, American Institute of Physics, 2017, 88 (5), pp.055106 〈10.1063/1.4982820〉
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https://hal.sorbonne-universite.fr/hal-01546067
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Soumis le : vendredi 23 juin 2017 - 13:02:57
Dernière modification le : mercredi 21 mars 2018 - 18:57:11

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T. Darnige, N. Figueroa-Morales, P. Bohec, A. Lindner, E. Clément. Lagrangian 3D tracking of fluorescent microscopic objects in motion. Review of Scientific Instruments, American Institute of Physics, 2017, 88 (5), pp.055106 〈10.1063/1.4982820〉. 〈hal-01546067〉

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