Circular oligomerization is an intrinsic property of synaptotagmin

Abstract : Previously, we showed that synaptotagmin1 (Syt1) forms Ca 2+-sensitive ring-like oligomers on membranes containing acidic lipids and proposed a potential role in regulating neurotransmitter release (Zanetti et al., 2016). Here, we report that Syt1 assembles into similar ring-like oligomers in solution when triggered by naturally occurring polyphosphates (PIP2 and ATP) and magnesium ions (Mg 2+). These soluble Syt1 rings were observed by electron microscopy and independently demonstrated and quantified using fluorescence correlation spectroscopy. Oligomerization is triggered when polyphosphates bind to the polylysine patch in C2B domain and is stabilized by Mg 2+ , which neutralizes the Ca 2+-binding aspartic acids that likely contribute to the C2B interface in the oligomer. Overall, our data show that ring-like polymerization is an intrinsic property of Syt1 with reasonable affinity that can be triggered by the vesicle docking C2B-PIP2 interaction and raise the possibility that Syt1 rings could pre-form on the synaptic vesicle to facilitate docking.
Type de document :
Article dans une revue
eLife, eLife Sciences Publication, 2017, 6, pp.e27441. 〈10.7554/eLife.27441〉
Liste complète des métadonnées

Littérature citée [42 références]  Voir  Masquer  Télécharger
Contributeur : Gestionnaire Hal-Upmc <>
Soumis le : lundi 18 septembre 2017 - 17:52:41
Dernière modification le : mardi 24 avril 2018 - 17:20:04


Publication financée par une institution


Distributed under a Creative Commons Paternité 4.0 International License




Jing Wang, Feng Li, Oscar D Bello, Charles Vaughn Sindelar, Frédéric Pincet, et al.. Circular oligomerization is an intrinsic property of synaptotagmin. eLife, eLife Sciences Publication, 2017, 6, pp.e27441. 〈10.7554/eLife.27441〉. 〈hal-01589630〉



Consultations de la notice


Téléchargements de fichiers