Fluorogenic Labeling Strategies for Biological Imaging

Abstract : The spatiotemporal fluorescence imaging of biological processes requires effective tools to label intracellular biomolecules in living systems. This review presents a brief overview of recent labeling strategies that permits one to make protein and RNA strongly fluorescent using synthetic fluorogenic probes. Genetically encoded tags selectively binding the exogenously applied molecules ensure high labeling selectivity, while high imaging contrast is achieved using fluorogenic chromophores that are fluorescent only when bound to their cognate tag, and are otherwise dark. Beyond avoiding the need for removal of unbound synthetic dyes, these approaches allow the development of sophisticated imaging assays, and open exciting prospects for advanced imaging, particularly for multiplexed imaging and super-resolution microscopy.
Type de document :
Article dans une revue
International Journal of Molecular Sciences, MDPI, 2017, 18 (7), pp.1473. 〈10.3390/ijms18071473〉
Liste complète des métadonnées

Littérature citée [74 références]  Voir  Masquer  Télécharger

https://hal.sorbonne-universite.fr/hal-01593560
Contributeur : Gestionnaire Hal-Upmc <>
Soumis le : mardi 26 septembre 2017 - 14:22:31
Dernière modification le : mardi 24 avril 2018 - 17:20:11
Document(s) archivé(s) le : mercredi 27 décembre 2017 - 13:34:58

Fichier

ijms-18-01473.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Chenge Li, Alison Tebo, Arnaud Gautier. Fluorogenic Labeling Strategies for Biological Imaging. International Journal of Molecular Sciences, MDPI, 2017, 18 (7), pp.1473. 〈10.3390/ijms18071473〉. 〈hal-01593560〉

Partager

Métriques

Consultations de la notice

110

Téléchargements de fichiers

48