Carbon evasion from rivers is an important component of the global carbon cycle. The intensification of anthropogenic pressures on hydrosystems requires studies of human-impacted rivers to identify and quantify the main drivers of carbon evasion. In 2016 and 2017, four field campaigns were conducted in the Seine River network characterized by an intensively cropped and highly populated basin. We measured partial pressures of carbon dioxide (pCO 2) in streams or rivers draining land under different uses at different seasons. We also computed pCO 2 from an existing data set (pH, water temperature and total alkalinity) going back until 1970. Here we report factors controlling pCO 2 that operate at different time and space scales. In our study, the Seine River was shown to be supersaturated in CO 2 with respect to the atmospheric equilibrium, as well as a source of CO 2. Our results suggest an increase in pCO 2 from winter to summer in small streams draining forests (from 1670 to 2480 ppm), croplands (from 1010 to 1550 ppm), and at the outlet of the basin (from 2490 to 3630 ppm). The main driver of pCO 2 was shown to be dissolved organic carbon (DOC) concentrations (R 2 = 0.56, n = 119, p < 0.05) that are modulated by hydro-climatic conditions and groundwater discharges. DOC sources were linked to land use and soil, mainly leaching into small upstream streams, but also to organic pollution, mainly found downstream in larger rivers. Our long-term analysis of the main stream suggests that pCO 2 closely mirrors the pattern of urban water pollution over time. These results suggest that factors controlling pCO 2 operate differently upstream and downstream depending on the physical characteristics of the river basin and on the intensity and location of the main anthropogenic pressures. The influence of these controlling factors may also differ over time, according to the seasons, and mirror long term changes in these anthropogenic pressures.