, Peptide Synthesis and Purification
SHa-COOH, FAKGIAGMAGKLF COOH ) were synthesized using solid-phase FastMoc chemistry on an Applied Biosystems 433A automated peptide synthesizer (Peptide Synthesis Platform ,
France) eluted at 0.75 mL/min by a 20-70% linear gradient of acetonitrile (1% ACN/min) in 0.1% TFA/water, followed by MALDI-TOF mass spectrometry analysis, C-terminal ?-carboxamidated peptides were prepared on a Rink Amide MBHA PS resin (Iris Biotech GmBH, Marktredwitz, Germany), whereas carboxylated peptides were prepared on a Fmoc-Phe-Wang resin LL, vol.2 ,
, Surface Preparation The gold substrates (i.e., gold-coated surfaces) are prepared following a standard protocol, vol.8
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, Samples of temporin analogs are available upon request and quotations from the authors through the IBPS synthesis platform. For further details please contact christophe