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Journal Articles Talanta Year : 2019

Validation of a global quantitative analysis methodology of tryptophan metabolites in mice using LC-MS

Abstract

In this study, we validated a method for quantifying 20 tryptophan (Trp) catabolites by liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) in 4 different matrices (urine, serum, intestinal contents and liver). The detection limit for all metabolites ranged between 0.015 and 11.25 nmol/L and the dynamic range of the calibration curves were adjusted to allow quantification of metabolites at endogenous levels. Matrix effects were evaluated using isotope labeled internal standards. Reproducibility in the 4 matrices was characterized by CV = 6.2% with an accuracy of 6.6%. Our method has been applied to the determination and quantification of 20 metabolites concentrations in 5 different mouse compartments (plus cecal contents). Our results show that our approach allows for a global exploration of the Trp metabolism by quantifying a large number of Trp metabolites, at the individual level by multi-matrix approach.
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Dates and versions

hal-02291264 , version 2 (18-09-2019)
hal-02291264 , version 1 (24-01-2023)
hal-02291264 , version 3 (10-11-2023)

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Antoine Lefèvre, Sylvie Mavel, Lydie Nadal-Desbarats, Laurent Galineau, Sylvie Attucci, et al.. Validation of a global quantitative analysis methodology of tryptophan metabolites in mice using LC-MS. Talanta, 2019, 195, pp.593-598. ⟨10.1016/j.talanta.2018.11.094⟩. ⟨hal-02291264v3⟩
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