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, examined using a transmission electron microscope (JEOL 1011) and photographed with a digital Erlangshen 1000 camera (GATAN)

, Electrocardiography| Electrocardiograms were recorded from mice using the non-invasive ecgTUNNEL (Emka Technologies) with minimal filtering. Waveforms were recorded using Iox Software and intervals were measured manually with ECG Auto. The electrocardiographer was blinded to mouse genotype

, Statistical significance between groups of mice analyzed by electrocardiography was analyzed with a corrected parametric test (Welch's t test), with a value of P<0.05 being considered significant. To validate results of echocardiographic analyses, we performed a non-parametric test (Wilcoxon-Mann-Whitney test). For all other experiments, a two-tailed Student's t test was used with a value of P<0.05 considered significant. Values are represented as means ± standard errors of mean (SEM). agglutinin labeling of cross-sections of hearts from 3 month, Statistics| Statistical analyses were performed using GraphPad Prism software

, Nuclei counter-stained with dapi are also shown. Data in pie graphs represent the quantification of lateralized Cx43 staining from n = 3 independent experiments. (C) Fluorescence micrographs showing Cx43 and a-tubulin labeling of isolated cardiomyocytes of hearts from 3 month-old male Lmna +/+ (WT) mice and Lmna, Lmna H222P/H222P (H222P) mice. Arrows indicate lateralization of Cx43

, H222P) mice. Arrows indicate lateralization of Cx43. Nuclei counter-stained with dapi are also shown. cardiac slices. (C) Quantification of lateral spreading from n = 3 independent experiments

, (WT) mice and Lmna H222P/H222P (H222P) mice. Nuclei counter-stained with dapi are also shown. (C) Immunoblot showing acetylated ?-tubulin expression from isolated cardiomyocytes of hearts from male Lmna +/+ (WT) mice and Lmna H222P/H222P (H222P) mice

, (WT) mice and isolated cardiomyocytes treated or not with nocodazole or taxol of hearts from 3 month-old male Lmna H222P/H222P (H222P) mice. Arrows indicate lateralization of Cx43

, Nuclei counter-stained with dapi are also shown. Graph showing percentage of lateralization of Cx43 from 5 month-old Lmna H222P/H222P (H222P) mice treated or not with Paclitaxel. Data are represented as means ± SEM (n = 150 cardiomyocytes from 3 mice per group

*. P&lt;0, 05). (D) Graphs showing mean QRS intervals in 5 month-old

, Data are represented as means ± standard errors of means. Values are shown as 25th to 75th percentiles of data values. The line in the middle is the median. Whiskers (Tukey method) extend down to the minimum value and up to the maximum value, Lmna H222P/H222P (H222P) mice treated with Paclitaxel or DMSO