FastQC: a quality control tool for high throughput sequence data, 2010. ,
, Bioinformatics. Available at
Controlling the false discovery rate: a practical and powerful 872 approach to multiple testing, J. Roy. Stat. Soc, vol.57, issue.1, pp.289-300, 1995. ,
Neurotransmitter-mediated control of 875 neurogenesis in the adult vertebrate brain, Development, vol.140, pp.2548-2561, 2013. ,
880 CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal 881 circuits, Nat Commun, vol.7, pp.1-8, 2016. ,
Trimmomatic: A flexible trimmer for Illumina sequence 883 data, Bioinformatics, vol.30, pp.2114-2120, 2014. ,
Mutations affecting 887 development of the midline and general body shape during zebrafish embryogenesis, Development, vol.888, pp.129-142, 1996. ,
The Reissner 890 Fiber in the Cerebrospinal Fluid Controls Morphogenesis of the Body Axis, Curr Biol, vol.891, pp.2479-2486, 2018. ,
Reissner fiber binds and 893 transports away monoamines present in the cerebrospinal fluid, Mol Brain Res, vol.894, pp.177-192, 2003. ,
Coordination of Morphogenesis and Cell-Fate 896 Specification in Development, Curr Biol, vol.27, 2017. ,
899 Regulation of the apical extension morphogenesis tunes the mechanosensory response 900 of microvilliated neurons, PLoS Biol, vol.17, pp.1-27, 2019. ,
The secretory material of the subcommissural organ 902 of the chick embryo. Characterization of a specific polypeptide by two-dimensional 903 electrophoresis, Int J Dev Biol, vol.39, pp.493-499, 1995. ,
, The 905, 1983.
, Subcommissural Organ-Liquor Fibre Complex: the Binding of Catecholamines to the 906 Liquor Fibre in Frogs of the Rana esculenta Complex, Acta Zool, vol.64, pp.47-53
The dual developmental 910 origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional 911 subtypes, Sci Rep, vol.7, pp.1-14, 2017. ,
, , p.913
STAR: Ultrafast universal RNA-seq aligner, Bioinformatics, vol.29, pp.15-914, 2013. ,
Emergence and Developmental Roles of the Cerebrospinal 916 Fluid System, Dev Cell, vol.52, pp.261-275, 2020. ,
Cilia-based flow network in the 918 brain ventricles, Science (80-), vol.353, pp.176-178, 2016. ,
State-dependent modulation of locomotion by GABAergic spinal sensory neurons, vol.920, 2015. ,
URL : https://hal.archives-ouvertes.fr/hal-01941112
, Curr Biol, vol.25, pp.3035-3047
Norepinephrine Diffusion from Hypothalamus to Cerebrospinal 923 Fluid and Cerebrospinal Fluid to Hypothalamus of Sheep, J Dairy Sci, vol.57, pp.826-832, 1974. ,
Localization of catecholamine uptake in rat brain after 926 intraventricular injection, Life Sci, vol.5, pp.1817-1824, 1966. ,
2017. ?2-Adrenergic 928 receptor activation mobilizes intracellular calcium via a non-canonical cAMP-929 independent signaling pathway, J Biol Chem, vol.292, pp.9967-9974 ,
, , 2016.
, Zebrafish models of idiopathic scoliosis link cerebrospinal fluid flow defects to spine 933 curvature, Science, vol.80, pp.1341-1344
Ciliogenesis and 935 cerebrospinal fluid flow in the developing Xenopus brain are regulated by foxj1, Cilia, vol.936, p.12, 2013. ,
Noradrenergic 938 neurons in the zebrafish hindbrain are induced by retinoic acid and require tfap2a for 939 expression of the neurotransmitter phenotype, Development, vol.130, pp.5741-5754, 2003. ,
, , p.942
, C21orf59/kurly Controls Both Cilia Motility 943 and Polarization, vol.14, pp.1841-1849, 2016.
,
WNT5A is transported via 947 lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis, Nat 948 Commun, vol.10, pp.1-15, 2019. ,
Stages of embryonic 950 development of the zebrafish, Dev Dyn, vol.203, pp.253-310, 1995. ,
Cilia-952 driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for 953 normal organogenesis, Development, vol.132, pp.1907-1921, 2005. ,
, WNT5a 955 Regulates Epithelial Morphogenesis in the Developing Choroid Plexus. Cereb Cortex 1-956 15, 2020.
Axon pathfinding and the floor plate factor Reissner's 958 substance in wildtype, cyclops and one-eyed pinhead mutants of Danio rerio, Dev Brain 959 Res, vol.154, pp.1-14, 2005. ,
, , p.961
, The Cerebrospinal, vol.962, 2011.
, Fluid Provides a Proliferative Niche for Neural Progenitor Cells, Neuron, vol.69, pp.893-905
Measurement of tritiated norepinephrine metabolism in 965 intact rat brain, J Neurosci Methods, vol.8, pp.155-160, 1983. ,
Reissner fibre-induced urotensin 967 signalling from cerebrospinal fluid-contacting neurons prevents scoliosis of the 968 vertebrate spine, 2020. ,
Brain norepinephrine and behavior. A behavioral and kinetic 970 study, Psychosom Med, vol.27, pp.399-407, 1965. ,
The 972 lengthening of a giant protein: When, how, and why?, J Mol Evol, vol.66, 2008. ,
Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and 976 Regulates Ventricular Development, Curr Biol, vol.975, pp.229-241, 2019. ,
, , p.979
Sensory Neurons Contacting 980 the Cerebrospinal Fluid Require the Reissner Fiber to Detect Spinal Curvature In Vivo, 2020. ,
, Curr Biol, vol.30, pp.827-839
Spatial and temporal regulation of ventral spinal cord 983 precursor specification by Hedgehog signaling, Development, vol.131, pp.5959-5969, 2004. ,
, , p.986
Comparative distribution and in vitro activities of the urotensin II-987 related peptides URP1 and URP2 in zebrafish: Evidence for their colocalization in spinal 988 cerebrospinal fluid-contacting neurons, PLoS One, vol.10, pp.1-21, 2015. ,
edgeR: A Bioconductor package for 991 differential expression analysis of digital gene expression data, Bioinformatics, vol.26, pp.139-992, 2009. ,
The retinal projectome reveals brain-area-specific visual 994 representations generated by ganglion cell diversity, Curr Biol, vol.24, pp.2085-2096, 2014. ,
, , p.997
Report SCO-Spondin Defects and 998 Neuroinflammation Are Conserved Mechanisms Driving Spinal Deformity across 999 Genetic Models of Idiopathic Scoliosis Report SCO-Spondin Defects and 1000 Neuroinflammation Are Conserved Mechanisms Driving Spinal Deformity across Gene 1001 1-11, 2020. ,
Genetic 1003 compensation induced by deleterious mutations but not gene knockdowns, Nature, vol.1004, pp.230-233, 2015. ,
, , p.1006
, , p.1007
Fiji: An open-source platform for biological-image 1008 analysis, Nat Methods, vol.9, pp.676-682, 2012. ,
, , p.1010
The Fz-Dsh Planar Cell Polarity Pathway Induces Oriented Cell 1011 Division via Mud/NuMA in Drosophila and Zebrafish, Dev Cell, vol.19, 2010. ,
Norepinephrine is required to promote 1014 wakefulness and for hypocretin-induced arousal in zebrafish, Elife, vol.4, pp.1-22, 1015. ,
In vivo and in vitro assessment of cardiac-adrenergic receptors in larval zebrafish 1018 (Danio rerio), J Exp Biol, vol.214, pp.1445-1457, 1017. ,
,
Pkd2l1 is required for mechanoception in 1022 cerebrospinal fluid-contacting neurons and maintenance of spine curvature, Nat 1023 Commun, vol.9, pp.1-10, 2018. ,
Zebrafish mutations affecting cilia motility share similar cystic phenotypes 1026 and suggest a mechanism of cyst formation that differs from pkd2 morphants, Dev Biol, vol.314, pp.261-275, 1027. ,
Origin and role of the cerebrospinal fluid 1030 bidirectional flow in the central canal, Elife, vol.9, pp.1-37, 2020. ,
Molecular evolution of GPCRs: Somatostatin/urotensin II receptors, J Mol 1033 Endocrinol, vol.52, 2014. ,
URL : https://hal.archives-ouvertes.fr/hal-02427066
, , p.1035
The Reissner Fiber is Highly Dynamic in Vivo and 1036 Controls Morphogenesis of the Spine. SSRN Electron J 1-10, 1037. ,
, , p.1039
International union of basic and clinical 1040 pharmacology. XCII. Urotensin II, urotensin II-related peptide, and their receptor: From 1041 structure to function, Pharmacol Rev, vol.67, pp.214-258, 2015. ,
, , p.1043
Zebrafish ?-adrenergic receptor mRNA expression and 1044 control of pigmentation, Gene, vol.446, pp.18-27, 2009. ,
Regulatory interactions specifying Kolmer-Agduhr 1046 interneurons, Development, vol.137, pp.2713-2722, 2010. ,
, , 1048.
, Cilia-driven cerebrospinal fluid flow directs expression of urotensin neuropeptides to 1049 straighten the vertebrate body axis, Nat Genet, vol.50, pp.1666-1673
, Figure 6 -Video 1. Adrb2 is expressed in the midline of the ventral most region of the 1170 neural tube that is different from that of CSF-contacting neurons
, Z-stack of a Tg(pkd2l1:GCaMP5G) control sibling (top) and a Tg
, scospondin icm15/icm15 mutant (bottom) at 30 hpf immunostained against GFP (green) and 1173
, In both cases, Adrb2-postive domains are inserted between two rows of 1174
, The video 1175 represents a 20.5 µm deep slice (0.5 µm z-step) for both embryos, GFP-positive ventral CSF-contacting neurons, pp.10-1176