We describe Microphorites pouilloni sp. nov., as first representative of this Cretaceous–Paleogene genus of Dolichopodidae Microphorinae from the mid-Cretaceous Burmese amber. The oldest records of this genus of small Diptera are from the Lower Cretaceous Lebanon and Jordan, while the Burmese amber was probably produced on an island during the mid-Cretaceous, which had separated from Gonwana during the Jusassic. This situation suggests a possible Late Jurassic origin for the genus Microphorites.