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Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons

Abstract : Plexin-B2 deletion leads to aberrant lamination of cerebellar granule neurons (CGNs) and Purkinje cells. Although in the cerebellum Plexin-B2 is only expressed by proliferating CGN precursors in the outer external granule layer (oEGL), its function in CGN development is still elusive. Here, we used 3D imaging, in vivo electroporation and live-imaging techniques to study CGN development in novel cerebellum-specific Plexin-B2 conditional knockout mice. We show that proliferating CGNs in Plexin-B2 mutants not only escape the oEGL and mix with newborn postmitotic CGNs. Furthermore, motility of mitotic precursors and early postmitotic CGNs is altered. Together, this leads to the formation of ectopic patches of CGNs at the cerebellar surface and an intermingling of normally time-stamped parallel fibers in the molecular layer (ML), and aberrant arborization of Purkinje cell dendrites. There results suggest that Plexin-B2 restrict CGN motility and might have a function in cytokinesis .
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https://hal.sorbonne-universite.fr/hal-03257970
Contributor : Hal Sorbonne Université Gestionnaire <>
Submitted on : Friday, June 11, 2021 - 11:23:29 AM
Last modification on : Sunday, June 13, 2021 - 3:26:03 AM

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Eljo van Battum, Celine Heitz-Marchaland, Yvrick Zagar, Stéphane Fouquet, Rohini Kuner, et al.. Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons. eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.60554⟩. ⟨hal-03257970⟩

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