Interaction of redox probes and ferrocene-labelled peptides with lipid bilayers observed at lipid bilayer-modified electrodes
Résumé
Glassy carbon electrodes (GCE) modified by a bilayer of phospholipids (BLM) have been used to monitor the passage of redox probes and ferrocene-labelled peptides across lipid membranes of an anionic (DOPG) or a zwitterionic (globally neutral DPhPC) phospholipid. The gel-phase neutral DPhPC showed a strong association with neutral probes like ferrocene methanol but expelled positively or negatively charged species, as observed on cyclic voltammograms. Anionic DOPG underwent a strong electrostatic interaction with the positively charged hexaamine ruthenium(III) complex, leading to aggregates that caused swelling and destructuration of the BLM. With positively charged ferrocenetagged peptides, DOPG-modified GCE allowed the observation of cyclic voltammograms exhibiting both a potential shift with respect to bare GCE and increased peak intensity, accounting for the slow but favored partition of the peptides between the BLM and the solution.
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