Amino-acyl tXNA as inhibitors or amino acid donors in peptide synthesis - Sorbonne Université
Article Dans Une Revue Nucleic Acids Research Année : 2022

Amino-acyl tXNA as inhibitors or amino acid donors in peptide synthesis

Résumé

Xenobiotic nucleic acids (XNAs) offer tremendous potential for synthetic biology, biotechnology, and molecular medicine but their ability to mimic nucleic acids still needs to be explored. Here, to study the ability of XNA oligonucleotides to mimic tRNA, we synthesized three L-Ala-tXNAs analogs. These molecules were used in a non-ribosomal peptide synthesis involving a bacterial Fem transferase. We compared the ability of this enzyme to use amino-acyl tXNAs containing 1′,5′-anhydrohexitol (HNA), 2′-fluoro ribose (2′F-RNA) and 2′-fluoro arabinose. L-Ala-tXNA containing HNA or 2′F-RNA were substrates of the Fem enzyme. The synthesis of peptidyl-XNA and the resolution of their structures in complex with the enzyme show the impact of the XNA on protein binding. For the first time we describe functional tXNA in an in vitro assay. These results invite to test tXNA also as substitute for tRNA in translation.
Fichier principal
Vignette du fichier
gkac1023.pdf (2.61 Mo) Télécharger le fichier
Origine Publication financée par une institution

Dates et versions

hal-03879768 , version 1 (05-12-2022)

Licence

Identifiants

Citer

Lauriane Rietmeyer, Inès Li de la Sierra-Gallay, Guy Schepers, Delphine Dorchêne, Laura Iannazzo, et al.. Amino-acyl tXNA as inhibitors or amino acid donors in peptide synthesis. Nucleic Acids Research, 2022, ⟨10.1093/nar/gkac1023⟩. ⟨hal-03879768⟩
69 Consultations
71 Téléchargements

Altmetric

Partager

More