Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro

Abstract : The objective of this project was to develop a bio-analytical approach leading to the assessment of the inherent hazard of the indoor air multi-contamination. Chromatographic methods combined with mass spectrometry were developed and validated for 62 target molecules known or suspected as endocrine-disrupting (ED) compounds. The ED potential was assessed by cellular bioassays measuring perturbations of transcriptional activity. The data showed that the predominant families of compounds in indoor air were in the following descendant order: phthalates > musks > alkylphenols > parabens. The ED contaminants were mainly present in gaseous phase, and the most contaminated locations were the day nursery and the house. An estrogenic, thyroid and anti-androgenic potential was attributed to indoor air. In agreement with its contamination profile, the biological activity of the latter was concentrated predominantly in the gaseous phase, and tended to be higher in the day nursery and the house. An effect-directed analysis (EDA) was carried out to identify the target chemicals responsible for the ED effects of indoor air. The following chemicals were identified as being potentially responsible for the observed ED effects: phthalates, methyl-paraben, alkylphenols, cypermethrin and synthetic musks. This work provides both knowledge about the inherent hazard of the indoor air multi-contamination and exposure data useful in health risk assessment.
Document type :
Theses
Complete list of metadatas

Cited literature [136 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02099667
Contributor : Abes Star <>
Submitted on : Monday, April 15, 2019 - 11:00:52 AM
Last modification on : Wednesday, May 15, 2019 - 3:39:26 AM

File

2015EPHE3059.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02099667, version 1

Citation

Stéphanie Laborie. Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro. Santé. École pratique des hautes études - EPHE PARIS, 2015. Français. ⟨NNT : 2015EPHE3059⟩. ⟨tel-02099667⟩

Share

Metrics

Record views

55

Files downloads

26