A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation
Huaying Zhao
(1)
,
Rodolfo Ghirlando
(1)
,
Carlos Alfonso
(2)
,
Fumio Arisaka
(3)
,
Ilan Attali
(4)
,
David L Bain
(5)
,
Marina M Bakhtina
(6)
,
Donald F Becker
(7)
,
Gregory J Bedwell
(8)
,
Ahmet Bekdemir
(9)
,
Tabot M D Besong
(10)
,
Catherine Birck
(11)
,
Chad A Brautigam
(12)
,
William Brennerman
(13)
,
Olwyn Byron
(14)
,
Agnieszka Bzowska
(15)
,
Jonathan B Chaires
(16)
,
Catherine T Chaton
(17)
,
Helmut Cölfen
(18)
,
Keith D Connaghan
(5)
,
Kimberly A Crowley
(19)
,
Ute Curth
(20)
,
Tina Daviter
(21, 22)
,
William L Dean
(16)
,
Ana I Díez
(23)
,
Christine Ebel
(24)
,
Debra M Eckert
(25)
,
Leslie E Eisele
(26)
,
Edward Eisenstein
(27)
,
Patrick England
(28)
,
Carlos Escalante
(29)
,
Jeffrey A Fagan
(30)
,
Robert Fairman
(31)
,
Ron M Finn
(32)
,
Wolfgang Fischle
(32)
,
José García de La Torre
(23)
,
Jayesh Gor
(22)
,
Henning Gustafsson
(33)
,
Damien Hall
(34)
,
Stephen E Harding
(10)
,
José G Hernández Cifre
(23)
,
Andrew B Herr
(35)
,
Elizabeth E Howell
(36)
,
Richard S Isaac
(37)
,
Shu-Chuan Jao
(38)
,
Davis Jose
(39)
,
Soon-Jong Kim
(40)
,
Bashkim Kokona
(31)
,
Jack A Kornblatt
(41)
,
Dalibor Kosek
(42)
,
Elena Krayukhina
(43)
,
Daniel Krzizike
(44)
,
Eric A Kusznir
(45)
,
Hyewon Kwon
(46)
,
Adam Larson
(37)
,
Thomas M Laue
(47)
,
Aline Le Roy
(24)
,
Andrew P Leech
(48)
,
Hauke Lilie
(49)
,
Karolin Luger
(44)
,
Juan R Luque-Ortega
(2)
,
Jia Ma
(1)
,
Carrie A May
(47)
,
Ernest L Maynard
(1)
,
Anna Modrak-Wojcik
(15)
,
Yee-Foong Mok
(50)
,
Norbert Mücke
(51)
,
Luitgard Nagel-Steger
(52)
,
Geeta J Narlikar
(37)
,
Masanori Noda
(43)
,
Amanda Nourse
(53)
,
Tomas Obsil
(42)
,
Chad K Park
(54)
,
Jin-Ku Park
(40)
,
Peter D Pawelek
(41)
,
Erby E Perdue
(13)
,
Stephen J Perkins
(22)
,
Matthew A Perugini
(55)
,
Craig L Peterson
(19)
,
Martin G Peverelli
(55)
,
Grzegorz Piszczek
(1)
,
Gali Prag
(4)
,
Peter E Prevelige
(8)
,
Bertrand D E Raynal
(28)
,
Lenka Rezabkova
(56)
,
Klaus Richter
(57)
,
Alison E Ringel
(58)
,
Rose Rosenberg
(18)
,
Arthur J Rowe
(59, 10)
,
Arne C Rufer
(45)
,
David J Scott
(60)
,
Javier G Seravalli
(7)
,
Alexandra S Solovyova
(61)
,
Renjie Song
(26)
,
David Staunton
,
Caitlin Stoddard
,
Katherine Stott
,
Holger M Strauss
(62)
,
Werner W Streicher
,
John P Sumida
,
Sarah G Swygert
,
Roman H Szczepanowski
,
Ingrid Tessmer
,
Ronald T Toth
(63)
,
Ashutosh Tripathy
(64)
,
Susumu Uchiyama
,
Stephan F W Uebel
,
Satoru Unzai
,
Anna Vitlin Gruber
(65)
,
Peter H von Hippel
,
Christine Wandrey
,
Szu-Huan Wang
(66)
,
Steven E Weitzel
,
Beata Wielgus-Kutrowska
,
Cynthia Wolberger
,
Martin Wolff
,
Edward Wright
(67)
,
Yu-Sung Wu
(68)
,
Jacinta M Wubben
,
Peter Schuck
(69)
1
NIH -
National Institutes of Health [Bethesda, MD, USA]
2 CSIC - Centro de Investigaciones Biológicas
3 Nihon University
4 TAU - Tel Aviv University
5 University of Colorado Anschutz [Aurora]
6 OSU - Ohio State University [Columbus]
7 University of Nebraska–Lincoln
8 UAB - University of Alabama at Birmingham [ Birmingham]
9 EPFL - Ecole Polytechnique Fédérale de Lausanne
10 UON - University of Nottingham, UK
11 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
12 University of Texas Southwestern Medical Center [Dallas]
13 Beckman Coulter Inc [Indianapolis, USA]
14 University of Glasgow
15 UW - University of Warsaw
16 University of Louisville
17 UC - University of Cincinnati
18 University of Konstanz
19 UMASS - University of Massachusetts Medical School [Worcester]
20 MHH - Medizinische Hochschule Hannover = Hannover Medical School
21 University of London [London]
22 UCL - University College of London [London]
23 Universidad de Murcia
24 UGA - Université Grenoble Alpes
25 University of Utah
26 New York State Department of Health [Albany]
27 University of Maryland System
28 Biophysique des macromolécules et leurs interactions
29 VCU - Virginia Commonwealth University
30 NIST - National Institute of Standards and Technology [Gaithersburg]
31 Haverford College
32 MPI-BPC - Max Planck Institute for Biophysical Chemistry
33 Novo Nordisk Inc
34 ANU - Australian National University
35 University of Cincinnati College of Medicine
36 The University of Tennessee [Knoxville]
37 UC San Francisco - University of California [San Francisco]
38 Academia Sinica
39 University of Oregon [Eugene]
40 Mokpo National University [Mokpo, South Korea]
41 Concordia University [Montreal]
42 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
43 U-Medico Inc. [Osaka, Japan]
44 CSU - Colorado State University [Fort Collins]
45 pRED - Roche Pharma Research and Early Development [Basel]
46 University of Washington [Seattle]
47 UNH - University of New Hampshire
48 University of York [York, UK]
49 Martin-Luther-University Halle-Wittenberg
50 University of Melbourne
51 DKFZ - German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg]
52 FZ Juelich
53 St Jude Children's Research Hospital
54 University of Arizona
55 La Trobe University [Melbourne]
56 PSI - Paul Scherrer Institute
57 TUM - Technische Universität Munchen - Technical University Munich - Université Technique de Munich
58 JHU - Johns Hopkins University
59 LPMC - Laboratoire de physique de la matière condensée
60 RAL - STFC Rutherford Appleton Laboratory
61 Newcastle University [Newcastle]
62 Institut für Geologie und Paläontologie
63 TU/e - Eindhoven University of Technology [Eindhoven]
64 Department of Biochemistry and Biophysics, UNC Macromolecular Interactions Facility
65 Institute of Botany
66 NSF Center for EUV Science and Technology
67 Sustainable Energy Economics
68 Department of mathematics and MOE-LSC
69 STLO - Science et Technologie du Lait et de l'Oeuf
2 CSIC - Centro de Investigaciones Biológicas
3 Nihon University
4 TAU - Tel Aviv University
5 University of Colorado Anschutz [Aurora]
6 OSU - Ohio State University [Columbus]
7 University of Nebraska–Lincoln
8 UAB - University of Alabama at Birmingham [ Birmingham]
9 EPFL - Ecole Polytechnique Fédérale de Lausanne
10 UON - University of Nottingham, UK
11 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
12 University of Texas Southwestern Medical Center [Dallas]
13 Beckman Coulter Inc [Indianapolis, USA]
14 University of Glasgow
15 UW - University of Warsaw
16 University of Louisville
17 UC - University of Cincinnati
18 University of Konstanz
19 UMASS - University of Massachusetts Medical School [Worcester]
20 MHH - Medizinische Hochschule Hannover = Hannover Medical School
21 University of London [London]
22 UCL - University College of London [London]
23 Universidad de Murcia
24 UGA - Université Grenoble Alpes
25 University of Utah
26 New York State Department of Health [Albany]
27 University of Maryland System
28 Biophysique des macromolécules et leurs interactions
29 VCU - Virginia Commonwealth University
30 NIST - National Institute of Standards and Technology [Gaithersburg]
31 Haverford College
32 MPI-BPC - Max Planck Institute for Biophysical Chemistry
33 Novo Nordisk Inc
34 ANU - Australian National University
35 University of Cincinnati College of Medicine
36 The University of Tennessee [Knoxville]
37 UC San Francisco - University of California [San Francisco]
38 Academia Sinica
39 University of Oregon [Eugene]
40 Mokpo National University [Mokpo, South Korea]
41 Concordia University [Montreal]
42 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
43 U-Medico Inc. [Osaka, Japan]
44 CSU - Colorado State University [Fort Collins]
45 pRED - Roche Pharma Research and Early Development [Basel]
46 University of Washington [Seattle]
47 UNH - University of New Hampshire
48 University of York [York, UK]
49 Martin-Luther-University Halle-Wittenberg
50 University of Melbourne
51 DKFZ - German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg]
52 FZ Juelich
53 St Jude Children's Research Hospital
54 University of Arizona
55 La Trobe University [Melbourne]
56 PSI - Paul Scherrer Institute
57 TUM - Technische Universität Munchen - Technical University Munich - Université Technique de Munich
58 JHU - Johns Hopkins University
59 LPMC - Laboratoire de physique de la matière condensée
60 RAL - STFC Rutherford Appleton Laboratory
61 Newcastle University [Newcastle]
62 Institut für Geologie und Paläontologie
63 TU/e - Eindhoven University of Technology [Eindhoven]
64 Department of Biochemistry and Biophysics, UNC Macromolecular Interactions Facility
65 Institute of Botany
66 NSF Center for EUV Science and Technology
67 Sustainable Energy Economics
68 Department of mathematics and MOE-LSC
69 STLO - Science et Technologie du Lait et de l'Oeuf
Catherine Birck
- Fonction : Auteur
- PersonId : 1145938
- IdHAL : catherine-birck
- ORCID : 0000-0003-1624-255X
Christine Ebel
- Fonction : Auteur
- PersonId : 1637
- IdHAL : christine-ebel
- ORCID : 0000-0002-6912-500X
- IdRef : 03372329X
Patrick England
- Fonction : Auteur
- PersonId : 735030
- IdHAL : patrick-england
- ORCID : 0000-0001-6410-5918
Grzegorz Piszczek
- Fonction : Auteur
- PersonId : 774961
- ORCID : 0000-0002-5270-3678
Bertrand D E Raynal
- Fonction : Auteur
- PersonId : 736286
- IdHAL : bertrand-raynal
- ORCID : 0000-0001-5634-0408
David Staunton
- Fonction : Auteur
Caitlin Stoddard
- Fonction : Auteur
Katherine Stott
- Fonction : Auteur
Werner W Streicher
- Fonction : Auteur
John P Sumida
- Fonction : Auteur
Sarah G Swygert
- Fonction : Auteur
Roman H Szczepanowski
- Fonction : Auteur
Ingrid Tessmer
- Fonction : Auteur
Susumu Uchiyama
- Fonction : Auteur
Stephan F W Uebel
- Fonction : Auteur
Satoru Unzai
- Fonction : Auteur
Peter H von Hippel
- Fonction : Auteur
Christine Wandrey
- Fonction : Auteur
Steven E Weitzel
- Fonction : Auteur
Beata Wielgus-Kutrowska
- Fonction : Auteur
Cynthia Wolberger
- Fonction : Auteur
Martin Wolff
- Fonction : Auteur
Jacinta M Wubben
- Fonction : Auteur
Résumé
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304 ± 0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of ± 0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies.
Domaines
Biologie structurale [q-bio.BM]| Origine | Fichiers éditeurs autorisés sur une archive ouverte |
|---|---|
| licence |
