Plasmodium sporozoites require the protein B9 to invade hepatocytes - Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé - UMS 37 PASS Access content directly
Journal Articles iScience Year : 2023

Plasmodium sporozoites require the protein B9 to invade hepatocytes

Abstract

Plasmodium sporozoites are transmitted to a mammalian host during blood feeding by an infected mosquito and invade hepatocytes for initial replication of the parasite into thousands of erythrocyte-invasive merozoites. Here we report that the B9 protein, a member of the 6-cysteine domain protein family, is secreted from sporozoite micronemes and is required for productive invasion of hepatocytes. The N-terminus of B9 forms a beta-propeller domain structurally related to CyRPA, a cysteine-rich protein forming an essential invasion complex in Plasmodium falciparum merozoites. The beta-propeller domain of B9 is essential for sporozoite infectivity and interacts with the 6-cysteine proteins P36 and P52 in a heterologous expression system. Our results suggest that, despite using distinct sets of parasite and host entry factors, Plasmodium sporozoites and merozoites may share common structural modules to assemble protein complexes for invasion of host cells.
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hal-03987907 , version 1 (14-02-2023)

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Priyanka Fernandes, Manon Loubens, Carine Marinach, Romain Coppe, Ludivine Baron, et al.. Plasmodium sporozoites require the protein B9 to invade hepatocytes. iScience, 2023, 26 (2), pp.106056. ⟨10.1016/j.isci.2023.106056⟩. ⟨hal-03987907⟩
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