A local subset of mesenchymal cells expressing the transcription factor Osr1 orchestrates lymph node initiation
Abstract
During development, lymph node (LN) initiation is coordinated by lymphoid tissue organizer (LTo) cells that attract lymphoid tissue inducer (LTi) cells at strategic positions within the embryo. The identity and function of LTo cells during the initial attraction of LTi cells remain poorly understood. Using lineage tracing, we demonstrated that a subset of Osr1-expressing cells was mesenchymal LTo progenitors. By investigating the heterogeneity of Osr1+ cells, we uncovered distinct mesenchymal LTo signatures at diverse anatomical locations, identifying a common progenitor of mesenchymal LTos and LN-associated adipose tissue. Osr1 was essential for LN initiation, driving the commitment of mesenchymal LTo cells independent of neural retinoic acid, and for LN-associated lymphatic vasculature assembly. The combined action of chemokines CXCL13 and CCL21 was required for LN initiation. Our results redefine the role and identity of mesenchymal organizer cells and unify current views by proposing a model of cooperative cell function in LN initiation.
Domains
Life Sciences [q-bio]
Fichier principal
Mesenchymal Osr1 cells orchestrate lymph node initiation_revised.pdf (8.32 Mo)
Télécharger le fichier
Origin : Files produced by the author(s)
licence : CC BY NC ND - Attribution - NonCommercial - NoDerivatives
licence : CC BY NC ND - Attribution - NonCommercial - NoDerivatives