Pulmonary toxoplasmosis in HIV-infected patients: usefulness of polymerase chain reaction and cell culture. - Sorbonne Université Access content directly
Journal Articles European Respiratory Journal Year : 1995

Pulmonary toxoplasmosis in HIV-infected patients: usefulness of polymerase chain reaction and cell culture.

I. Lavrard
  • Function : Author
J. L. Poirot
  • Function : Author
B. Lemarchand
  • Function : Author
M. C. Meyohas
  • Function : Author
J. L. Olivier
  • Function : Author

Abstract

Toxoplasmosis is a serious opportunistic infection in patients with acquired immunodeficiency syndrome (AIDS). The lung is a major site of infection after the central nervous system. The aim of the study was to assess the polymerase chain reaction (PCR) and cell culture for the detection of Toxoplasma gondii. One hundred and thirty two human immunodeficiency virus (HIV)-infected patients with respiratory manifestations, who underwent fibreoptic bronchoalveolar lavage, were investigated. Detection of Toxoplasma gondii was compared using three techniques: Giemsa staining; polymerase chain reaction with specific primers derived from the P30 gene; and culture on the MRC5 cell line. Toxoplasma gondii was detected in the same four samples by all three techniques. We conclude that PCR adds little to conventional (and cheaper) tools already used to diagnose pulmonary toxoplasmosis.
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Dates and versions

hal-00597500 , version 1 (01-06-2011)

Identifiers

  • HAL Id : hal-00597500 , version 1
  • PUBMED : 7544741

Cite

I. Lavrard, C. Chouaid, P. Roux, J. L. Poirot, M. Marteau, et al.. Pulmonary toxoplasmosis in HIV-infected patients: usefulness of polymerase chain reaction and cell culture.. European Respiratory Journal, 1995, 8 (5), pp.697-700. ⟨hal-00597500⟩
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