Bioconjugated gold nanorods to enhance the sensitivity of FT-SPR-based biosensors - Sorbonne Université Access content directly
Journal Articles Colloids and Surfaces B: Biointerfaces Year : 2012

Bioconjugated gold nanorods to enhance the sensitivity of FT-SPR-based biosensors

Abstract

n this paper, we report a feasible solvent-free approach for the synthesis and self-assembling of gold nanorods after bioconjugation to antibodies; scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed a remarkable shape and size narrow distribution, as well as a tendency to form linear assemblies on a clean gold surface, upon interaction with antibodies. These bioconjugated gold nanorods were in turns tested in a model Fourier Transform Surface Plasmon Resonance (FT-SPR)-based immunosensor, leading to an improvement of the detection sensitivity by a factor of 8. The results highlight the simplicity of the synthesis protocol of gold nanorods and the interest of using them as labels to enhance the sensitivity of SPR-based sensors.

Domains

Catalysis

Dates and versions

hal-00744490 , version 1 (23-10-2012)

Identifiers

Cite

Jolanda Spadavecchia, Sandra Casale, Souhir Boujday, Claire-Marie Pradier. Bioconjugated gold nanorods to enhance the sensitivity of FT-SPR-based biosensors. Colloids and Surfaces B: Biointerfaces, 2012, 100, pp.1-8. ⟨10.1016/j.colsurfb.2012.03.035⟩. ⟨hal-00744490⟩
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