DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant? - Sorbonne Université Access content directly
Journal Articles Sensors and Actuators B: Chemical Year : 2012

DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?

Abstract

The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k(2) (s(-1)), relevant to irreversible formation of a stable duplex, varies in an opposite way to K-1 with DNA target length.

Dates and versions

hal-00789976 , version 1 (19-02-2013)

Identifiers

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Mathieu Lazerges, Hubert Perrot, N. Rabehagasoa, Chantal Compère, C. Dreanno, et al.. DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?. Sensors and Actuators B: Chemical, 2012, 171-172, pp.522-527. ⟨10.1016/j.snb.2012.05.023⟩. ⟨hal-00789976⟩
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