Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts - Sorbonne Université Access content directly
Journal Articles Surface and Interface Analysis Year : 2011

Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts

Abstract

Poly(oligoethylene glycol methacrylate), POEGMA, brushes were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated silicon wafers. Prior to ATRP, the substrates were grafted by brominated aryl initiators via the electrochemical reduction of a noncommercial parent diazonium salt of the formula BF4-, N-+(2)-C6H4-CH(CH3)Br. The diazonium-modified gold plates (Au-Br) served as macroinitiators for ATRP of OEGMA which resulted in hydrophilic surfaces (Au-POEGMA) that could be used for two distinct objectives: (i) resistance to fouling by Salmonella Typhimurium; (ii) specific recognition of the same bacteria provided that the POEGMA grafts are activated by anti-Salmonella. The Au-POEGMA plates were characterized by XPS, polarization modulation-infrared reflection-absorption spectroscopy (PM-IRRAS) and contact angle measurements. Both Beer-Lambert equation and Tougaard's QUASES software indicated a POEGMA thickness that exceeds the critical similar to 10 nm value necessary for obtaining a hydrophilic polymer with effective resistance to cell adhesion. The Au-POEGMA slides were further activated by trichlorotriazine (TCT) in order to covalently bind anti-Salmonella antibodies (AS). The antibody-modified Au-POEGMA specimens were found to specifically attach Salmonella Typhimurium bacteria. This work is another example of the diazonium salt/ATRP process to provide biomedical polymer surfaces.

Dates and versions

hal-00830461 , version 1 (05-06-2013)

Identifiers

Cite

Bechir Mrabet, Aymen Mejbri, Samia Mahouche, Sarra Gam-Derouich, Mireille Turmine, et al.. Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts. Surface and Interface Analysis, 2011, 43 (11), pp.1436-1443. ⟨10.1002/sia.3741⟩. ⟨hal-00830461⟩
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