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Article Dans Une Revue Journal of Peptide Science Année : 2014

Temporin-SHa peptides grafted on gold surfaces display antibacterial activity

Résumé

Development of resistant bacteria onto biomaterials is a major problem leading to nosocomial infections. Antimicrobial peptides are good candidates for the generation of antimicrobial surfaces because of their broad-spectrum activity and their original mechanism of action (i.e. rapid lysis of the bacterial membrane) making them less susceptible to the development of bacterial resistance. In this study, we report on the covalent immobilisation of temporin-SHa on a gold surface modified by a thiolated self-assembledmonolayer. Temporin-SHa (FLSGIVGMLGKLFamide) is a small hydrophobic and low cationic antimicrobial peptide with potent and very broad-spectrum activity against Gram-positive and Gram-negative bacteria, yeasts and parasites. We have analysed the influence of the binding mode of temporin-SHa on the antibacterial efficiency by using a covalent binding either via the peptide NH2 groups (random grafting of α- and ε-NH2 to the surface) or via its C-terminal end (oriented grafting using the analogue temporin-SHa-COOH). The surface functionalization was characterised by IR spectroscopy (polarisation modulation reflection absorption IR spectroscopy) while antibacterial activity against Listeria ivanovii was assessed by microscopy techniques, such as atomic force microscopy and scanning electron microscopy equipped with a field emission gun. Our results revealed that temporin-SHa retains its antimicrobial activity after covalent grafting. A higher amount of bound temporin-SHa is observed for the C-terminally oriented grafting compared with the random grafting (NH2 groups). Temporin-SHa therefore represents an attractive candidate as antimicrobial coating agent. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.

Dates et versions

hal-01044512 , version 1 (23-07-2014)

Identifiants

Citer

Andres Lombana, Zahid Raja, Sandra Casale, Claire-Marie Pradier, Thierry Foulon, et al.. Temporin-SHa peptides grafted on gold surfaces display antibacterial activity. Journal of Peptide Science, 2014, 20, pp.563-569. ⟨10.1002/psc.2654⟩. ⟨hal-01044512⟩
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