Solid state NMR and diatoms: probing the interfaces - Sorbonne Université
Conference Poster Year : 2014

Solid state NMR and diatoms: probing the interfaces

Abstract

Studies on silica biomineralization focus principally on marine sponges and diatoms. Diatoms are known to synthesise a silicified cell wall named “frustule” by intracellular transport and polymerisation of silicic acid Si(OH)4. Frustule is also composed of organic matter - mainly polysaccharides, proteins such as silaffins and long chain polyamines - that were already demonstrated to be capable of inducing silica polymerization into nanospheres of ca. 50-200 nm in diameter. In this context, solid-state Nuclear Magnetic Resonance (ssNMR) appears to be a powerful tool for studying the mineral-organics interactions in Thalassiosira pseudonana specimen. To do so, isotopically doubly-enriched (15N, 13C) T. Pseudonana were studied as whole cells, SDS-extracted and H2O2-cleaned samples by 1H, 13C, 15N, 29Si and 31P ssNMR through 1D and 2D experiments.
Fichier principal
Vignette du fichier
Poster biomineralization_SMa_final.pdf (1.47 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-01138970 , version 1 (03-04-2015)

Licence

Identifiers

  • HAL Id : hal-01138970 , version 1

Cite

Sylvie Masse, Guillaume Laurent, T. Coradin. Solid state NMR and diatoms: probing the interfaces. Biomineralization short course, Dec 2014, Paris, France. . ⟨hal-01138970⟩
238 View
423 Download

Share

More