Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles - Sorbonne Université Access content directly
Journal Articles Molecular Therapy - Methods and Clinical Development Year : 2015

Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles

Régis Gayon
  • Function : Author
Christine Duthoit
  • Function : Author
Nicolas Martin
Raphaël Sevrain
  • Function : Author
Lucille Lamouroux
  • Function : Author
Frédéric Deschaseaux
  • Function : Author
Pascale Bouillé
  • Function : Author
Luc Sensébé
  • Function : Author

Abstract

RNA delivery is an attractive strategy to achieve transient gene expression in research projects and in cell- or gene-based therapies. Despite significant efforts investigating vector-directed RNA transfer, there is still a requirement for better efficiency of delivery to primary cells and in vivo. Retroviral platforms drive RNA delivery, yet retrovirus RNA-packaging constraints limit gene transfer to two genome-molecules per viral particle. To improve retroviral transfer, we designed a dimerization-independent MS2-driven RNA packaging system using MS2-Coat-retrovirus chimeras. The engineered chimeric particles promoted effective packaging of several types of RNAs and enabled efficient transfer of biologically active RNAs in various cell types, including human CD34+ and iPS cells. Systemic injection of high-titer particles led to gene expression in mouse liver and transferring Cre-recombinase mRNA in muscle permitted widespread editing at the ROSA26 locus. We could further show that the VLPs were able to activate an osteoblast differentiation pathway by delivering RUNX2- or DLX5-mRNA into primary human bone-marrow mesenchymal-stem cells. Thus, the novel chimeric MS2-lentiviral particles are a versatile tool for a wide range of applications including cellular-programming or genome-editing.
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hal-01219159 , version 1 (22-10-2015)

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Anne Prel, Vincent Caval, Régis Gayon, Philippe Ravassard, Christine Duthoit, et al.. Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles. Molecular Therapy - Methods and Clinical Development, 2015, 2, pp.15039. ⟨10.1038/mtm.2015.39⟩. ⟨hal-01219159⟩
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