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HJURP Involvement in De Novo CenH3CENP-A and CENP-C Recruitment

Abstract : Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3CENP-A in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3CENP-A contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores. We find that de novo CENP-T accumulation depends on CENP-C and that recruitment of these factors requires two domains in CenH3CENP-A: the HJURP-binding region (CATD) and the CENP-C-binding region (CAC). Notably, HJURP interacts directly with CENP-C and is critical for de novo accumulation of CENP-C at synthetic centromeres. On the basis of our findings, we propose that HJURP serves a dual chaperone function in coordinating CenH3CENP-A and CENP-C recruitment.
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Submitted on : Wednesday, November 4, 2015 - 10:38:37 AM
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Hiroaki Tachiwana, Sebastian Müller, Julia Blümer, Kerstin Klare, Andrea Musacchio, et al.. HJURP Involvement in De Novo CenH3CENP-A and CENP-C Recruitment. Cell Reports, Elsevier Inc, 2015, 11 (1), pp.22-32. ⟨10.1016/j.celrep.2015.03.013⟩. ⟨hal-01224138⟩

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