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A multiscale study of bacterial proliferation modes within novel E. coli@Si(HIPE) hybrid macrocellular living foams

Abstract : For the first time the study at various length scales of E. coli proliferation modes within Si(HIPE) inorganic macrocellular foams is proposed. Both qualitatively and semi-quantitatively, the bacterial proliferation within the foam is not homogeneous and is directly linked to the Si(HIPE)'s macroscopic cells random distribution. When inoculated in a nutrients loaded Si(HIPE), the bacterial growth is enhanced within the Si(HIPE) matrices compared to the surrounding LB media. The bacteria growth kinetics tends to be faster and the concentration at saturation is roughly 100% times higher. In the case of a Si(HIPE) host free of nutrients, the bacterial motion is occurring as an infiltration wave, the peak of this propagation wave moving at a constant speed of 88 µm h-1 , while bacterial concentrations within the Si(HIPE) reach levels far above the ones reached with the presence of nutrients, suggesting a real synergetic relation between the bacterial colony guests and the Si(HIPE) host. When a nutrients reservoir is present at the opposite position from which bacteria were inoculated, the bacterial proliferation is associated to a coalescence process between the growing colonies that were rapidly established within the first hours. When the Si(HIPE) is fully colonized we found out a specific distance between adjacent colonies of 5 to 15 µm in good correspondence with the repartition of the wall to wall distances of the Si(HIPE)'s macroscopic cells, meaning that the bacterial repartition once the colonization occurred is optimum. These results show that Si(HIPE) foams represent outstanding candidates for strengthened bacterial proliferation without the motion restriction imposed by conventional silica gels.
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Submitted on : Monday, March 14, 2016 - 5:05:17 PM
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Martin Depardieu, Mélanie Viaud, Axel Buguin, Jacques Livage, Clément Sanchez, et al.. A multiscale study of bacterial proliferation modes within novel E. coli@Si(HIPE) hybrid macrocellular living foams. Journal of Materials Chemistry B: Materials for Biology and Medicine, Royal Society of Chemistry, 2016, 4, pp.2290-2303. ⟨10.1039/C5TB02554K⟩. ⟨hal-01288191⟩

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