HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Remote z-scanning with a macroscopic voice coil motor for fast 3D multiphoton laser scanning microscopy

Abstract : There is a high demand for 3D multiphoton imaging in neuroscience and other fields but scanning in axial direction presents technical challenges. We developed a focusing technique based on a remote movable mirror that is conjugate to the specimen plane and translated by a voice coil motor. We constructed cost-effective z-scanning modules from off-the-shelf components that can be mounted onto standard multiphoton laser scanning microscopes to extend scan patterns from 2D to 3D. Systems were designed for large objectives and provide high resolution, high speed and a large z-scan range (>300 μm). We used these systems for 3D multiphoton calcium imaging in the adult zebrafish brain and measured odor-evoked activity patterns across >1500 neurons with single-neuron resolution and high signal-to-noise ratio.
Document type :
Journal articles
Complete list of metadata

https://hal.sorbonne-universite.fr/hal-01327206
Contributor : Gestionnaire 2 Hal-Upmc Connect in order to contact the contributor
Submitted on : Monday, June 6, 2016 - 2:57:34 PM
Last modification on : Saturday, May 21, 2022 - 3:41:42 AM

File

boe-7-5-1656.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Peter Rupprecht, Andrew Prendergast, Claire Wyart, Rainer W. Friedrich. Remote z-scanning with a macroscopic voice coil motor for fast 3D multiphoton laser scanning microscopy. Biomedical optics express, Optical Society of America - OSA Publishing, 2016, 7 (5), pp.1656-1671. ⟨10.1364/BOE.7.001656⟩. ⟨hal-01327206⟩

Share

Metrics

Record views

52

Files downloads

140