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Conference papers

Ex vivo characterization of calcium pyrophosphate-based osteoarticular calcifications by X-ray diffraction and Raman spectroscopy

Abstract : Although several techniques have been used for the characterization of ex vivo calcium pyrophosphate (CPP: Ca2P2O7·nH2O) deposits associated to osteoarthritis,1–4 little is known from a chemical point of view on these phases which are difficult to synthesize, could evolve during their formation in vivo and be altered after extraction during conservation, preparation and analysis. Recently, Gras et al. established a protocol allowing a one-step and fast synthesis of four phases of CPP of biological interest by monitoring the pH and temperature during the synthesis.5 The stability and evolution properties of these synthetic phases have been investigated and ex vivo specimen analyses were conducted on cryoground meniscii and synovial fluid specimens of arthritic patients to avoid any alteration of the samples. Synchrotron X-ray diffraction and Raman spectroscopy were used to analyse synthetic samples, including monoclinic and triclinic calcium pyrophosphate dihydrate (m-CPPD and t-CPPD: Ca2P2O7·2H2O), the two phases detected in joints of arthritic patients, and ex vivo biological samples. This study highlights the good selectivity of these techniques to detect both of the CPPD phases in complex media such as meniscus and synovial fluid, and to determine their ratio. In accordance with their thermodynamic stability, the data suggests the evolution of CPP in vivo from m-CPPD to t-CPPD. Although precursor phases could be involved their identification seems yet difficult. Progresses in the fine characterization of the different synthetic CPP phases could improve their detection in patients suffering from calcium-salt crystal diseases and could contribute to clarifying the mechanism by which CPP crystals form and evolve in vivo.
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Conference papers
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https://hal.sorbonne-universite.fr/hal-01329185
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Wednesday, June 8, 2016 - 5:52:35 PM
Last modification on : Wednesday, September 23, 2020 - 4:35:12 AM

Identifiers

  • HAL Id : hal-01329185, version 1

Citation

Pierre Gras, Hang Korng Ea, Olivier Marsan, Laure Campillo-Gimenez, Kemie Ley-Ngardigal, et al.. Ex vivo characterization of calcium pyrophosphate-based osteoarticular calcifications by X-ray diffraction and Raman spectroscopy. 50th Annual Scientific Meeting of the European Society for Clinical Investigation Paris, Apr 2016, Paris, France. pp.8.12. ⟨hal-01329185⟩

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