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Model of the delayed translation of cyclin B maternal mRNA after sea urchin fertilization

Abstract : Sea urchin eggs exhibit a cap-dependent increase in protein synthesis within minutes after fertilization. This rise in protein synthesis occurs at a constant rate for a great number of proteins translated from the different available mRNAs. Surprisingly, we found that cyclin B, a major cell-cycle regulator, follows a synthesis pattern that is distinct from the global protein population, so we developed a mathematical model to analyze this dissimilarity in biosynthesis kinetic patterns. The model includes two pathways for cyclin B mRNA entry into the translational machinery: one from immediately available mRNA (mRNAcyclinB) and one from mRNA activated solely after fertilization (XXmRNAcyclinB). Two coefficients, α and β, were added to fit the measured scales of global protein and cyclin B synthesis, respectively. The model was simplified to identify the synthesis parameters and to allow its simulation. The calculated parameters for activation of the specific cyclin B synthesis pathway after fertilization included a kinetic constant (ka) of 0.024 sec−1, for the activation of XXmRNAcyclinB, and a critical time interval (t2) of 42 min. The proportion of XXmRNAcyclinB form was also calculated to be largely dominant over the mRNAcyclinB form. Regulation of cyclin B biosynthesis is an example of a select protein whose translation is controlled by pathways that are distinct from housekeeping proteins, even though both involve the same cap-dependent initiation pathway. Therefore, this model should help provide insight to the signaling utilized for the biosynthesis of cyclin B and other select proteins. Mol. Reprod. Dev. 2016. © 2016 Wiley Periodicals, Inc.
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Vincent Picard, Odile Mulner-Lorillon, Jérémie Bourdon, Julia Morales, Patrick Cormier, et al.. Model of the delayed translation of cyclin B maternal mRNA after sea urchin fertilization. Molecular Reproduction and Development, Wiley, 2016, ⟨10.1002/mrd.22746⟩. ⟨hal-01390047⟩

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