Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins - Sorbonne Université
Journal Articles Chemistry - A European Journal Year : 2016

Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins

Sami Jannin

Abstract

Hyperpolarization is generated by dissolution dynamic nuclear polarization (d-DNP) using a polymer-based polarizing agent dubbed FLAP (filterable labeled agents for polarization). It consists of a thermo-responsive poly(N-isopropylacrylamide), also known as pNiPAM-COOH, labeled with nitroxide radicals. The polymer powder is impregnated with an arbitrary solution of interest and frozen as is. Dissolution is followed by a simple filtration, leading to hyperpolarized solutions free from any contaminants. We demonstrated the use of FLAP to hyperpolarize partially deuterated water up to P(1H)=6 % with a long relaxation T1 >36 s characteristic of high purity. Water hyperpolarization can be transferred to drugs, metabolites, or proteins that are waiting in an NMR spectrometer, either by exchange of labile protons or through intermolecular Overhauser effects. We also show that FLAPs are suitable polarizing agents for 13C-labeled metabolites such as pyruvate, acetate, and alanine.

Dates and versions

hal-01498642 , version 1 (30-03-2017)

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Basile Vuichoud, Aurélien Bornet, Florian De nanteuil, Jonas Milani, Estel Canet, et al.. Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins. Chemistry - A European Journal, 2016, 22 (41), pp.14696 - 14700. ⟨10.1002/chem.201602506⟩. ⟨hal-01498642⟩
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