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Glucosomes: Glycosylated Vesicle-in-Vesicle Aggregates in Water from pH-Responsive Microbial Glycolipid

Abstract : Vesicle-in-vesicle self-assembled containers, or vesosomes, are promising alternatives to liposomes because of their possible hierarchical encapsulation and high stability. We report herein the first example of sugar-based vesicles-in-vesicles, which we baptize glucosomes. These were prepared by using a natural microbial glycolipid (branched C22 sophorolipid) extracted from the culture medium of the yeast Pseudohyphozyma bogoriensis. Glucosomes spontaneously formed in water between pH 6 and pH 4 at room temperature, without the requirement of any additive. By means of pH-resolved in situ small angle X-ray scattering, we provided direct evidence for the vesicle-formation mechanism. Statistical treatment of the vesicle radii distribution measured by cryo-tansmission electron microscopy by using a derived form of the Helfrich bending free-energy expression provided an order of magnitude for the effective bending constant (the sum of the curvature and the saddle-splay moduli) of the lipid membrane to K=(0.4±0.1) kBT. This value is in agreement with the bending constant measured for hydrocarbon-based vesicles membranes.
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Submitted on : Tuesday, August 29, 2017 - 11:07:48 AM
Last modification on : Monday, December 14, 2020 - 9:44:13 AM


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Niki Baccile, Patrick Le griel, Sylvain Prévost, Bernd Everaert, Wim Soetaert, et al.. Glucosomes: Glycosylated Vesicle-in-Vesicle Aggregates in Water from pH-Responsive Microbial Glycolipid. ChemistryOpen, Wiley, 2017, 6 (4), pp.526-533. ⟨10.1002/open.201700101⟩. ⟨hal-01578430⟩



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