A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation

Abstract : The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body. This magnetic approach to embryoid body formation has no discernible impact on ESC differentiation pathways, as compared to the hanging drop method. It is also the base of the final magnetic device, composed of opposing magnetic attractors in order to form embryoid bodies in situ, then stretch them, and mechanically stimulate them at will. These stretched and cyclic purely mechanical stimulations were sufficient to drive ESCs differentiation towards the mesodermal cardiac pathway.
Type de document :
Article dans une revue
Nature Communications, Nature Publishing Group, 2017, 8, pp.400. 〈10.1038/s41467-017-00543-2〉
Liste complète des métadonnées

Littérature citée [70 références]  Voir  Masquer  Télécharger

https://hal.sorbonne-universite.fr/hal-01593593
Contributeur : Gestionnaire Hal-Upmc <>
Soumis le : mardi 26 septembre 2017 - 14:43:38
Dernière modification le : mercredi 21 mars 2018 - 18:58:23
Document(s) archivé(s) le : mercredi 27 décembre 2017 - 13:14:06

Fichier

s41467-017-00543-2.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Vicard Du, Nathalie Luciani, Sophie Richard, Gaëtan Mary, Cyprien Gay, et al.. A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation. Nature Communications, Nature Publishing Group, 2017, 8, pp.400. 〈10.1038/s41467-017-00543-2〉. 〈hal-01593593〉

Partager

Métriques

Consultations de la notice

244

Téléchargements de fichiers

57