Id genes are essential for early heart formation

Abstract : Deciphering the fundamental mechanisms controlling cardiac specification is critical for our understanding of how heart formation is initiated during embryonic development and for applying stem cell biology to regenerative medicine and disease modeling. Using systematic and unbiased functional screening approaches, we discovered that the Id family of helix–loop–helix proteins is both necessary and sufficient to direct cardiac mesoderm formation in frog embryos and human embryonic stem cells. Mechanistically, Id proteins specify cardiac cell fate by repressing two inhibitors of cardiogenic mesoderm formation—Tcf3 and Foxa2—and activating inducers Evx1, Grrp1, and Mesp1. Most importantly, CRISPR/Cas9-mediated ablation of the entire Id (Id1–4) family in mouse embryos leads to failure of anterior cardiac progenitor specification and the development of heartless embryos. Thus, Id proteins play a central and evolutionarily conserved role during heart formation and provide a novel means to efficiently produce cardiovascular progenitors for regenerative medicine and drug discovery applications.
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Genes and Development, Cold Spring Harbor Laboratory Press, 2017, 31 (13), pp.1325-1338. 〈10.1101/gad.300400.117〉
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Soumis le : mardi 26 septembre 2017 - 16:10:27
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Thomas Cunningham, Michael Yu, Wesley Mckeithan, Sean Spiering, Florent Carrette, et al.. Id genes are essential for early heart formation. Genes and Development, Cold Spring Harbor Laboratory Press, 2017, 31 (13), pp.1325-1338. 〈10.1101/gad.300400.117〉. 〈hal-01593675〉



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