HGF potentiates extracellular matrix-driven migration of human myoblasts: involvement of matrix metalloproteinases and MAPK/ERK pathway

Abstract : Background: The hepatocyte growth factor (HGF) is required for the activation of muscle progenitor cells called satellite cells (SC), plays a role in the migration of proliferating SC (myoblasts), and is present as a soluble factor during muscle regeneration, along with extracellular matrix (ECM) molecules. In this study, we aimed at determining whether HGF is able to interact with ECM proteins, particularly laminin 111 and fibronectin, and to modulate human myoblast migration. Methods: We evaluated the expression of the HGF-receptor c-Met, laminin, and fibronectin receptors by immunoblotting, flow cytometry, or immunofluorescence and used Transwell assays to analyze myoblast migration on laminin 111 and fibronectin in the absence or presence of HGF. Zymography was used to check whether HGF could modulate the production of matrix metalloproteinases by human myoblasts, and the activation of MAPK/ERK pathways was evaluated by immunoblotting. Results: We demonstrated that human myoblasts express c-Met, together with laminin and fibronectin receptors. We observed that human laminin 111 and fibronectin have a chemotactic effect on myoblast migration, and this was synergistically increased when low doses of HGF were added. We detected an increase in MMP-2 activity in myoblasts treated with HGF. Conversely, MMP-2 inhibition decreased the HGF-associated stimulation of cell migration triggered by laminin or fibronectin. HGF treatment also induced in human myoblasts activation of MAPK/ERK pathways, whose specific inhibition decreased the HGF-associated stimulus of cell migration triggered by laminin 111 or fibronectin. Conclusions: We demonstrate that HGF induces ERK phosphorylation and MMP production, thus stimulating human myoblast migration on ECM molecules. Conceptually, these data state that the mechanisms involved in the migration of human myoblasts comprise both soluble and insoluble moieties. This should be taken into account to optimize the design of therapeutic cell transplantation strategies by improving the migration of donor cells within the host tissue, a main issue regarding this approach.
Type de document :
Article dans une revue
Skeletal Muscle, BioMed Central, 2017, 7 (1), pp.20. 〈10.1186/s13395-017-0138-6〉
Liste complète des métadonnées

Littérature citée [86 références]  Voir  Masquer  Télécharger

https://hal.sorbonne-universite.fr/hal-01622802
Contributeur : Gestionnaire Hal-Upmc <>
Soumis le : mardi 24 octobre 2017 - 16:43:04
Dernière modification le : lundi 8 octobre 2018 - 17:44:09
Document(s) archivé(s) le : jeudi 25 janvier 2018 - 15:07:20

Fichier

s13395-017-0138-6.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

UPMC | CEA | RIIP

Citation

Mariela González, Wallace De Mello, Gillian S. Butler-Browne, Suse Dayse Silva-Barbosa, Vincent Mouly, et al.. HGF potentiates extracellular matrix-driven migration of human myoblasts: involvement of matrix metalloproteinases and MAPK/ERK pathway. Skeletal Muscle, BioMed Central, 2017, 7 (1), pp.20. 〈10.1186/s13395-017-0138-6〉. 〈hal-01622802〉

Partager

Métriques

Consultations de la notice

216

Téléchargements de fichiers

51