Cocaine increases dopaminergic connectivity in the nucleus accumbens

Abstract : The development of addictive behavior is associated with functional and structural plasticity in the mesocorticolimbic pathway. Increased connectivity upon cocaine administration has been inferred from increases in dendritic spine density, but without observations of presynaptic elements. Recently, we established a method that enables analyses of both dendritic spines and glutamatergic boutons and presented evidence that cocaine induces changes in striatal connectivity. As the pharmacological and behavioral effects of cocaine directly implicate dopaminergic neurons and their afferents, a remaining question is whether dopaminergic striatal innervations also undergo structural plasticity. To address this issue, we generated transgenic mice in which the fluorophore tdTomato is expressed under the promoter of the dopamine transporter gene. In these mice, specific labeling of dopaminergic boutons was observed in the striatum. Of note, the accordance of our results for control mice with previous electron microscopy studies confirms that our method can be used to decipher the spatial organization of boutons in relation to dendritic elements. Following repeated cocaine administration that led to behavioral locomotor sensitization, an increased density of dopaminergic boutons was observed one day later in the nucleus accumbens shell specifically, and not in other striatal regions. Combined labeling of dopaminergic boutons and striatal dendrites showed that cocaine significantly increased the percentage of dendritic spines associated with a dopaminergic bouton. Our results show that chronic cocaine Dos Santos et al. 2 administration induces structural plasticity of dopaminergic boutons that could participate in dopamine-dependent neuronal adaptations in the striatum.
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Brain Structure and Function, Springer Verlag, 2017, 〈10.1007/s00429-017-1532-x〉
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Soumis le : lundi 30 octobre 2017 - 15:47:04
Dernière modification le : vendredi 31 août 2018 - 08:57:21
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Marc Santos, Emma Cahill, Gregory Dal Bo, Peter Vanhoutte, Jocelyne Caboche, et al.. Cocaine increases dopaminergic connectivity in the nucleus accumbens. Brain Structure and Function, Springer Verlag, 2017, 〈10.1007/s00429-017-1532-x〉. 〈hal-01626292〉



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