Extracellular versus Intracellular Degradation of Nanostructured Silica Particles - Sorbonne Université Accéder directement au contenu
Article Dans Une Revue Langmuir Année : 2018

Extracellular versus Intracellular Degradation of Nanostructured Silica Particles

Christophe Hélary
T. Coradin

Résumé

Silica nanoparticles appear as promising drug carriers for intracellular delivery. However, the mechanisms by which they are degraded within cells remain largely unknown. In this context, we have prepared three types of PEGylated fluorescent silica nanoparticles with various internal structures (core–shell biocomposite, multilayered, and hollow mesoporous) and studied their degradation in a buffer, in a culture medium, and in contact with human dermal fibroblasts. All particles were prone to dissolve in solution, leading to an increase of porosity and/or the precipitation of new colloids and eventually fragmentation, with a faster rate in the medium compared to that in the buffer. All particles were also uptaken by the cells without significant cytotoxic effect. Their intracellular degradation occurred faster than in suspension, but following almost similar dissolution mechanisms. These results strongly suggest that in these conditions, silica nanoparticles must be primarily considered as hydrolytically degraded and not biodegraded, a point of importance for their future applications in drug delivery.

Domaines

Chimie
Fichier principal
Vignette du fichier
Shi et al. - 2017 - Extracellular vs. Intracellular Degradation of Nan.pdf (679.94 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01668691 , version 1 (20-12-2017)

Identifiants

Citer

Yupeng Shi, Christophe Hélary, Bernard Haye, T. Coradin. Extracellular versus Intracellular Degradation of Nanostructured Silica Particles. Langmuir, 2018, 34 (1), pp.406-415. ⟨10.1021/acs.langmuir.7b03980⟩. ⟨hal-01668691⟩
57 Consultations
209 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More