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Article Dans Une Revue Nature Communications Année : 2017

Identification of an analgesic lipopeptide produced by the probiotic Escherichia coli strain Nissle 1917

Claude Knauf
Jean Claude Tabet
Gilles Dietrich
Nicolas Cénac
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Résumé

Administration of the probiotic Escherichia coli strain Nissle 1917 (EcN) decreases visceral pain associated with irritable bowel syndrome. Mutation of clbA, a gene involved in the biosynthesis of secondary metabolites, including colibactin, was previously shown to abrogate EcN probiotic activity. Here, we show that EcN, but not an isogenic clbA mutant, produces an analgesic lipopeptide. We characterize lipoamino acids and lipopeptides produced by EcN but not by the mutant by online liquid chromatography mass spectrometry. One of these lipopeptides, C12AsnGABAOH, is able to cross the epithelial barrier and to inhibit calcium flux induced by nociceptor activation in sensory neurons via the GABA B receptor. C12AsnGABAOH inhibits visceral hypersensitivity induced by nociceptor activation in mice. Thus, EcN produces a visceral analgesic, which could be the basis for the development of new visceral pain therapies.
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hal-01873396 , version 1 (13-09-2018)

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Teresa Pérez-Berezo, Julien Pujo, Patricia Martin, Pauline Le Faouder, Jean-Marie Galano, et al.. Identification of an analgesic lipopeptide produced by the probiotic Escherichia coli strain Nissle 1917. Nature Communications, 2017, 8 (1), pp.8: 1314. ⟨10.1038/s41467-017-01403-9⟩. ⟨hal-01873396⟩
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