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Journal Articles Biomedical optics express Year : 2018

In vivo laser Doppler holography of the human retina


The eye offers a unique opportunity for the non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome this limitation, we used digital holography to perform laser Doppler perfusion imaging of human retina with near-infrared light. Two imaging channels with a slow and a fast CMOS camera were used simultaneously for real-time narrowband measurements, and offline wideband measurements, respectively. The beat frequency spectrum of optical interferograms recorded with the fast (up to 75 kHz) CMOS camera was analyzed by short-time Fourier transformation. Power Doppler images drawn from the Doppler power spectrum density qualitatively revealed blood flow in retinal vessels over 512 × 512 pixels covering 2.4 × 2.4 mm 2 on the retina with a temporal resolution down to 1.6 ms. The sensitivity to lateral motion as well as the requirements in terms of sampling frequency are discussed.
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Dates and versions

hal-01875560 , version 1 (17-09-2018)





L. Puyo, M. Paques, M. Fink, José-Alain Sahel, Michael Atlan. In vivo laser Doppler holography of the human retina. Biomedical optics express, 2018, 9 (9), pp.4113-4129. ⟨10.1364/BOE.9.004113⟩. ⟨hal-01875560⟩
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