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Clathrin plaques and associated actin anchor intermediate filaments in skeletal muscle

Abstract : Clathrin plaques are stable features of the plasma membrane observed in several cell types. They are abundant in muscle, where they localize at costameres that link the contractile apparatus to the sarcolemma and connect the sarcolemma to the basal lamina. Here, we show that clathrin plaques and surrounding branched actin filaments form microdomains that anchor a three-dimensional desmin intermediate filament (IF) web. Depletion of clathrin plaque and branched actin components causes accumulation of desmin tangles in the cytoplasm. We show that dynamin 2, whose mutations cause centronuclear myopathy (CNM), regulates both clathrin plaques and surrounding branched actin filaments, while CNM-causing mutations lead to desmin disorganization in a CNM mouse model and patient biopsies. Our results suggest a novel paradigm in cell biology, wherein clathrin plaques act as platforms capable of recruiting branched cortical actin, which in turn anchors IFs, both essential for striated muscle formation and function.
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https://hal.sorbonne-universite.fr/hal-02136143
Contributor : Hal Sorbonne Université Gestionnaire <>
Submitted on : Tuesday, May 21, 2019 - 6:32:28 PM
Last modification on : Thursday, September 2, 2021 - 9:08:50 AM

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Agathe Franck, Jeanne Lainé, Gilles Moulay, Eline Lemerle, Michaël Trichet, et al.. Clathrin plaques and associated actin anchor intermediate filaments in skeletal muscle. Molecular Biology of the Cell, American Society for Cell Biology, 2019, 30 (5), pp.579-590. ⟨10.1091/mbc.E18-11-0718⟩. ⟨hal-02136143⟩

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