Skip to Main content Skip to Navigation
Journal articles

Proline oxidation fuels mitochondrial respiration during dark-induced leaf senescence in Arabidopsis thaliana

Abstract : Leaf senescence is a form of developmentally programmed cell death that allows the remobilization of nutrients and cellular materials from leaves to sink tissues and organs. Among the catabolic reactions that occur upon senescence, little is known about the role of proline catabolism. In this study, the involvement in dark-induced senescence of pro-line dehydrogenases (ProDHs), which catalyse the first and rate-limiting step of proline oxidation in mitochondria, was investigated using prodh single-and double-mutants with the help of biochemical, proteomic, and metabolomic approaches. The presence of ProDH2 in mitochondria was confirmed by mass spectrometry and immunogold labelling in dark-induced leaves of Arabidopsis. The prodh1 prodh2 mutant exhibited enhanced levels of most tricarb-oxylic acid cycle intermediates and free amino acids, demonstrating a role of ProDH in mitochondrial metabolism. We also found evidence of the involvement and the importance of ProDH in respiration, with proline as an alternative substrate, and in remobilization of proline during senescence to generate glutamate and energy that can then be exported to sink tissues and organs.
Complete list of metadata

Cited literature [62 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-02430967
Contributor : Gestionnaire Hal-Su <>
Submitted on : Tuesday, January 7, 2020 - 4:17:27 PM
Last modification on : Monday, December 14, 2020 - 9:53:05 AM

File

erz351.pdf
Publication funded by an institution

Identifiers

Citation

Alban Launay, Cecile Cabassa-Hourton, Holger Eubel, Régis Maldiney, Anne Guivarc'h, et al.. Proline oxidation fuels mitochondrial respiration during dark-induced leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (21), pp.6203-6214. ⟨10.1093/jxb/erz351⟩. ⟨hal-02430967⟩

Share

Metrics

Record views

142

Files downloads

315