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Investigating CaOx Crystal Formation in Absence and Presence of Polyphenols under Microfluidic Conditions in Relation with Nephrolithiasis

Abstract : Nephrolithiasis is a major health concern in western countries. Herein, we propose a microfluidic based approach to mimic the physical and physicochemical conditions encountered in the collecting duct in a nephron where calcium oxalate (CaOx) precipitation occurs. Our objective is to understand the parameters involved in the formation of such crystals. The microfluidic platform is reversible, allowing interfacial characterizations using scanning electron microscopy imaging and Raman spectroscopy. CaOx crystalline phases and morphologies were studied with respect to hydrodynamics and physicochemical conditions within the channel and at the outlet. While calcium oxalate monohydrate (COM) crystals were dominant within the channel, at the outlet, the crystals were calcium oxalate dihydrate (COD) crystals, which agrees with medical observations. Decreasing the flow rate lowered down the induction time for CaOx formation and enhanced the occurrence of COD crystals. The kinetics of COM crystals growth studied in situ showed two regimes, an initial surface-limited reaction, followed by a transport-limited growth with a dependency of the kinetics on the position of the crystal in the channel. Numerical modeling of CaOx formation in a microchannel using an in-house model considering the chemical reactions involved allowed to confirm the experimental observations on the location of precipitate formation but also to quantitatively match the scaling law related to the early growth of precipitate particles. Finally, the effect of polyphenols naturally found in green tea (GT) on modulating CaOx crystallization was studied in the microfluidic device in different scenarios where GT was initially mixed in solution with the Ca and/or the Ox precursors. The formation of COD crystals rather than COM ones was always predominant; however, depending on the conditions, CaOx crystals of different morphologies could be observed, including COD crystals with an elongated (100) crystalline face and COM crystals with a round-shaped morphology with a concave crystalline face.
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Submitted on : Tuesday, October 27, 2020 - 12:44:03 PM
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Karol Rakotozandriny, Samantha Bourg, Paszkál Papp, Ágota Tóth, Dezső Horváth, et al.. Investigating CaOx Crystal Formation in Absence and Presence of Polyphenols under Microfluidic Conditions in Relation with Nephrolithiasis. Crystal Growth & Design, American Chemical Society, 2020, pp.7683-7693. ⟨10.1021/acs.cgd.0c00929⟩. ⟨hal-02979866⟩



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